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. 2022 Sep 22;11(10):1876. doi: 10.3390/antiox11101876

Figure 3.

Figure 3

(A) SELENOP relative gene expression and (B) SELENOP protein expression in macrophages cultured in the presence (+) or absence (−) of FBS for 6 or 24 h and (C) SELENOP relative gene expression in macrophages maintained for 24 h in (+)FBS or in (−)FBS and supplemented with 12.5 and 625 μM OH-SeMet for an additional 72 h. Gene expression results were calculated using 2−ΔCt. The results are expressed as mean ± SEM of n = 6 cultures. The asterisk (*) denotes significant differences (p < 0.05) between (+)FBS and (−)FBS (A and B) and the letters denote differences (p < 0.05) between incubation periods (A) and conditions (C).