. 2022 Oct 20;11(10):2069. doi: 10.3390/antiox11102069

Table 3.

Cu ²⁺ chelating activity of lichen extracts and quercetin as the reference.

Extracts/ Substance	Chelating Cu²⁺ [%]						IC₅₀ [µg/mL]
	Concentration [mg/mL]
	0.005	0.01	0.02	0.04	0.08	0.16
DCM extract	18.7 ± 0.2 ^b	35.1 ± 1.2 ^b	58.8 ± 2.0 ^b	88.4 ± 2.6 ^b	96.0 ± 0.8 ^a	94.7 ± 1.2 ^ab	14.7 ± 0.6 ^a
Ace extract	26.3 ± 1.5 ^a	43.7 ± 0.6 ^a	71.4 ± 0.3 ^a	91.2 ± 1.3 ^ab	96.9 ± 0.5 ^a	96.1 ± 0.3 ^ab	12.0 ± 0.8 ^a
MeOH extract	nt	25.3 ± 1.4 ^c	38.7 ± 4.6 ^c	59.2 ± 4.4 ^c	94.7 ± 0.5 ^a	96.7 ± 0.1 ^ab	29.8 ± 2.4 ^ba
MeOH-H₂O extract	nt	17.3 ± 0.1 ^d	28.2 ± 1.3 ^d	46.7 ± 1.6 ^d	72.3 ± 3.6 ^b	90.3 ± 0.7 ^b	45.7 ± 3.1 ^b
H₂O extracta	nt	10.5 ± 0.2 ^e	16.8 ± 1.8 ^e	25.4 ± 3.1 ^e	43.6 ± 0.9 ^c	51.1 ± 1.6 ^c	141.0 ± 17.4 ^c
Quercetin	26.0 ± 2.0 ^a	44.3 ± 0.5 ^a	72.7 ± 0.9 ^a	97.5 ± 1.0 ^a	97.7 ± 0.1 ^a	98.5 ± 5.2 ^a	12.4 ± 0.4 ^a

Mean values within a column with the same letter are not significantly different at p 0.05 using Duncan’s test. The first letter of the alphabet for the highest values, the next for statistically significant decreasing values. The mean values ± SEM from n = 4 independent measurements; nt—not tested at this concentration.