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. 2022 Oct 20;11(10):2069. doi: 10.3390/antiox11102069

Table 4.

Fe 2+ chelating activity of lichen extracts and quercetin as the reference.

Extracts/
Substance
Chelating Fe2+ [%] IC50
[mg/mL]
Concentration [mg/mL]
0.08 0.16 0.8 1.6 4.0
DCM extract 7.9 ± 0.3 c 13.8 ± 0.7 c 35.6 ± 0.8 d 51.7 ± 3.6 c 61.4 ± 1.3 c 1.83 ± 0.21 d
Ace extract 6.5 ± 0.1 dc 7.0 ± 1.3 d 20.7 ± 0.2 e 36.6 ± 5.6 d 40.0 ± 3.2 d 4.0 e
MeOH extract 10.9 ± 0.5 b 16.9 ± 1.5 c 52.9 ± 2.7 b 79.3 ± 2.2 b 96.1 ± 0.3 a 0.55 ± 0.02 b
MeOH-H2O extract 36.0 ± 1.3 a 64.2 ± 1.8 a 90.9 ± 2.4 a 98.0 ± 0.2 a 100.2 ± 0.9 a 0.10 ± 0.01 a
H2O extract 34.4 ± 0.9 a 48.2 ± 2.5 b 92.8 ± 0.5 a 96.5 ± 0.3 d 99.2 ± 0.5 a 0.15 ± 0.01 a
Quercetin 5.2 ± 0.9 d 16.2 ± 0.8 c 42.5 ± 1.0 c 54.2 ± 1.7 c 72.6 ± 1.8 b 1.17 ± 0.03 c

Mean values within a column with the same letter are not significantly different at p 0.05 using Duncan’s test. The first letter of the alphabet for the highest values, the next for statistically significant decreasing values. The mean values ± SEM from n = 4 independent measurements (extracts) or from n = 6 independent measurements (quercetin).