Figure 1.

Concentration-dependent effects of plant extracts on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) and inhibitory activities. A total of 18 plant extracts were screened using both AChE and BuChE inhibitory assays. Di-nitro-thiobenzoate was added to wells of a 96-well plate, followed by either acetylthiocholine iodide (ATCI) or butyrylthiocholine chloride (BTCC) as substrates. Phytoextracts were tested in duplicate at concentrations ranging from 25–500 µg/mL. Mean percentage inhibition data is summarised in the heatmaps. Positive control: galantamine (18 µM in AChE experiments or 2 µM in BuChE experiments); negative control: sodium phosphate buffer.