Table 1.
Controls | Screened Plant Extract (n = 5) ± Std. Error | |||||||
---|---|---|---|---|---|---|---|---|
Kinetics Properties | NC | PC | J. r | E. c | C. z | S. o | H. p | |
AChE | Vmax (mM/min) | 0.008651 ± 0.0005743 | 0.00869 ± 0.0004418 | 0.00849 ± 0.0003554 | 0.003548 ± 0.0002209 | 0.004507 ± 0.0004567 | 0.00434 ± 0.0004683 | 0.00469 ± 0.0004768 |
Km (mM) |
0.003523 ± 0.001149 | 0.01441 ± 0.002464 | 0.01092 ± 0.001659 | 0.001344 ± 0.0006181 | 0.01951 ± 0.006144 | 0.01644 ± 0.005772 | 0.00246 ± 0.001398 | |
Proposed Inhibitory type | N/A | competitive | competitive | mixed | mixed | mixed | uncompetitive | |
BuChE | Vmax (mM/min) | 0.00753 ± 0.0007588 | 0.00565 ± 0.001249 | 0.00391 ± 0.0004516 | 0.002214 ± 0.0002146 | 0.005219 ± 0.0008111 | 0.00372 ± 0.0003888 | 0.00383 ± 0.0006241 |
Km (mM) |
0.09534 ± 0.03577 | 0.43380 ± 0.2367 | 0.0938 ± 0.04096 | 0.02058 ± 0.01200 | 0.3486 ± 0.1430 | 0.14000 ± 0.04964 | 0.09475 ± 0.05747 | |
Proposed Inhibitory type | N/A | mixed | uncompetitive | mixed | mixed | mixed | uncompetitive |
Vmax, Km, and the proposed type of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities are mentioned. Kinetic studies involved fixed concentrations of the Galantamine inhibitor (18 µM for AChE or 2 µM for BuChE) or screened phytoextracts (n = 5; 500 µg/mL) and variable substrate concentrations (i.e., acetylthiocholine iodide (ATCI): 0.0023–0.15 mM for AchE inhibition assay or butyrilthiocholine chloride (BTCC): 0.023–1.5 mM for BuChE inhibition assay). Km and Vmax parameters were calculated by Michaelis–Menten curve fitting. N/A = not applicable. NC = negative control (no inhibitor); PC = positive control (Galantamine). J. r: Juglan regia; E. c: Ellettaria cardamomum; C. z: Cinnamomum zeylanicum; S. o: Salvia officinalis; H. p: Hypericum perforatum.