Table 1.

AChE and BuChE inhibitory properties of selected plant extracts.

		Controls		Screened Plant Extract (n = 5) ± Std. Error
	Kinetics Properties	NC	PC	J. r	E. c	C. z	S. o	H. p
AChE	Vmax (mM/min)	0.008651 ± 0.0005743	0.00869 ± 0.0004418	0.00849 ± 0.0003554	0.003548 ± 0.0002209	0.004507 ± 0.0004567	0.00434 ± 0.0004683	0.00469 ± 0.0004768
	Km (mM)	0.003523 ± 0.001149	0.01441 ± 0.002464	0.01092 ± 0.001659	0.001344 ± 0.0006181	0.01951 ± 0.006144	0.01644 ± 0.005772	0.00246 ± 0.001398
	Proposed Inhibitory type	N/A	competitive	competitive	mixed	mixed	mixed	uncompetitive
BuChE	Vmax (mM/min)	0.00753 ± 0.0007588	0.00565 ± 0.001249	0.00391 ± 0.0004516	0.002214 ± 0.0002146	0.005219 ± 0.0008111	0.00372 ± 0.0003888	0.00383 ± 0.0006241
	Km (mM)	0.09534 ± 0.03577	0.43380 ± 0.2367	0.0938 ± 0.04096	0.02058 ± 0.01200	0.3486 ± 0.1430	0.14000 ± 0.04964	0.09475 ± 0.05747
	Proposed Inhibitory type	N/A	mixed	uncompetitive	mixed	mixed	mixed	uncompetitive

Vmax, Km, and the proposed type of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities are mentioned. Kinetic studies involved fixed concentrations of the Galantamine inhibitor (18 µM for AChE or 2 µM for BuChE) or screened phytoextracts (n = 5; 500 µg/mL) and variable substrate concentrations (i.e., acetylthiocholine iodide (ATCI): 0.0023–0.15 mM for AchE inhibition assay or butyrilthiocholine chloride (BTCC): 0.023–1.5 mM for BuChE inhibition assay). Km and Vmax parameters were calculated by Michaelis–Menten curve fitting. N/A = not applicable. NC = negative control (no inhibitor); PC = positive control (Galantamine). J. r: Juglan regia; E. c: Ellettaria cardamomum; C. z: Cinnamomum zeylanicum; S. o: Salvia officinalis; H. p: Hypericum perforatum.