Table 2.
BACE-1 inhibitory property of selected plant extracts (n = 4).
| Controls | Screened Plant Extract (n = 4) | ||||||
|---|---|---|---|---|---|---|---|
| Kinetics Properties | NC | PC | J. r | E. c | F. t | S. o | |
| BACE-1 | Vmax (mM/min) |
361.9 | 361.7 | 276.5 | 351.0 | 361.3 | 120.3 |
| Km (mM) |
2.881 | 6.062 | 6.893 | 4.627 | 4.82 | 0.9230 | |
| Proposed Inhibitory type |
N/A | competitive | mixed | mixed | competitive | uncompetitive | |
| IC50 (mg/mL) | N/A | 25 µM | 0.55 | 0.60 | 0.81 | 0.53 | |
Vmax, Km, the proposed type of the β-secretase inhibitory activity, and IC50 values are mentioned. Kinetic studies involved fixed concentrations of the KF-14 inhibitor (25 µM) or phytoextract (n = 4; 500 µg/mL) and variable substrate concentrations (0.156–10 mM). Km and Vmax parameters were calculated by Michaelis–Menten curve fitting. N/A = not applicable. NC = negative control (no inhibitor); PC = positive control (KF-14). J. r: Juglan regia; E. c: Ellettaria cardamomum; F. t: Cinnamomum zeylanicum; S. o: Salvia officinalis.