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. 2022 Oct 12;13:1002616. doi: 10.3389/fimmu.2022.1002616

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Copyright © 2022 Lv, Zhang, Zhao, Yang, Zou, Zhao, Li, Sun, Lin and Jin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression C1QTNF3 inhibits poly (dA:dT)-induced inflammatory cytokine production in PK-15 cells. (A, B) Overexpression of HA-tagged C1QTNF3 proteins was detected using western blot analysis. The mRNA levels of (C) IL-6 and (D) IL-8 in PK-15 cells with C1QTNF3 overexpression for 24 h followed by poly (dA:dT) stimulation for 48 h. The GAPDH gene was used as the internal control for normalization. The 2^-ΔΔCt method was used to analyze the results of qPCR. The protein levels of (E) IL-8 and (F) IL-6 in PK-15 cells with C1QTNF3 overexpression for 24 h followed by poly (dA:dT) stimulation for 48 h p <0.05 (*), p < 0.01 (**), and p < 0.001 (***).