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. 2022 Oct 12;13:1002616. doi: 10.3389/fimmu.2022.1002616

Figure 8.

Figure 8

Knockdown of C1QTNF3 increases poly (dA:dT)-induced inflammatory cytokine production in PK-15 cells. (A, B) Detection of C1QTNF3 siRNA knockdown efficiency using western blot analysis. The mRNA levels of (C) IL-6 and (D) IL-8 in PK-15 cells with C1QTNF3 downregulation for 24 h followed by poly (dA:dT) stimulation for 48 h. The GAPDH gene was used as the internal control for normalization. The 2-ΔΔCt method was used to analyze the results of qPCR. The protein levels of (E) IL-8 and (F) IL-6 in PK-15 cells with C1QTNF3 downregulation for 24 h followed by poly (dA:dT) stimulation for 48 h p <0.05 (*), p < 0.01 (**).