NRF2 protein expression is upregulated in high grade dysplasia and esophageal adenocarcinoma. (A) Western blotting analysis of NRF2 protein expression in cell lines originated from the normal esophageal squamous epithelium (HEEC), Barrett’s esophagus (BAR10T and CPA), dysplastic Barrett’s (CPB) and esophageal adenocarcinoma (FLO1, ESO26, ESO51, OE33, and OE19). (B) Immunohistochemistry analysis of the NRF2 protein expression in primary human tissues of normal esophagus (NE), Barrett’s esophagus (BE), and esophageal adenocarcinoma (EAC), showing overexpression in EAC. Representative images of 100× (upper panels) and 400× (lower panels) are shown. (C,D) A summary of NRF2 IHC staining scores in NE, BE, HGD, and EAC from the tissue microarrays, showing high levels of NRF2 in HGD and EAC tissues (the chi-square test, p < 0.0001).