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. 2022 Sep 21;11(10):1859. doi: 10.3390/antiox11101859

Figure 4.

Figure 4

Brusatol inhibited NRF2 activity and killed EAC cells at low concentrations. (A) The ARE (antioxidant response element) luciferase reporter assay indicates the inhibition of NRF2 ARE activity at a 50–100 nM concentration of Brusatol. (B) Real-time RT-PCR shows the downregulation of NRF2 downstream target genes, HO1 and GR after Brusatol treatment. (C) Western blot displays the downregulation of the HO1 protein level after Brusatol treatment in the EAC cells. (D–G) The CellTiter Glo cell viability assay indicates that EAC cells were sensitive to Brusatol treatment with an IC50 below 100 nM (D,E), whereas the Barrett’s esophagus cells (F) and normal esophageal fibroblast cells (G) were more resistant to Brusatol treatment. The blue dot lines in D-G indicate the crosspoints between the 50% survival line and the dose-response curve.