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. 2022 Sep 21;11(10):1859. doi: 10.3390/antiox11101859

Figure 7.

Figure 7

Brusatol synergized with CDDP in killing tumor cells in vivo. (A) Representative images of xenogrfting tumors after 30 days of treatments of Brusatol, CDDP, or Brusatol + CDDP. (B) The tumor growth curve shows that Brusatol and CDDP alone significantly slowed tumor growth, but the Brusatol and CDDP combination generated the most suppressive effect. (C) Dual immunohistochemistry staining of the cell proliferation marker (Ki-67, brown) and apoptosis marker (cleaved caspase 3, magenta) in the xenografted tumor tissues. The quantitative data using ImageJ software are shown in (E) (Ki-67) and (F) (cleaved caspase 3). (D) Immunohistochemistry staining of ferroptosis marker, GPX4, in xenografted tumor tissues shows the significant downregulation of GPX4. The quantitative data using ImageJ software are shown in (G). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.