Table 7.
Tolerance to acidic pH and 0.3% bile salts evaluated by the viability of LABS4 and LABS14 strains isolated from rainbow trout intestinal content, incubated for 4 h at 37 °C at pH 3.0 or in the presence of 0.3% (w/v) bile salts.
| LAB Strain | Acid Resistance | Bile Salts Resistance | ||
|---|---|---|---|---|
| pH 3 (Viability %) | Control | Bile Salt (Viability %) | Control | |
| LABS4 | * 8 × 108 ± 0.06 (57.1) | 14 × 108 ± 0.12 | * 9 × 108 ± 0.07 (69.2) | 13 × 108 ± 0.18 |
| LABS14 | * 12 × 108 ± 0.10 (74.8) | 16 × 108 ± 0.09 | * 12 × 108 ± 0.14 (82.3) | 15 × 108 ± 0.03 |
Acid resistance: N° of CFU mL−1; Bile resistance: N° of CFU mL−1. Results are shown as viable cells percentage when compared to cells not subjected to HCl or bile salts (control) assigned as 100%. * Significantly smaller than corresponding control (p < 0.05). Data is given as mean ± SD; Experiments were carried out in triplicate.