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. 2022 Oct 1;11(10):1973. doi: 10.3390/antiox11101973

Figure 8.

Figure 8

Confocal images of intracellular ROS generation upon platelet-derived growth factor (PDGF) stimulation in live HLE cells. Live HLE cells were preloaded with DCFH-DA (50 μM) to capture the ROS generated upon PDGF (1 ng/mL) stimulation. Confocal cell images represent a random field after PDGF exposure. (A) PDGF-stimulated cells at 10 min. (B) Non-PDGF-stimulated cells at 10 min. (C) Catalase (I mg/mL) preloaded cells at 10 min after PDGF exposure. (D) Inhibition of PDGF-stimulated ROS generation by antioxidants and free radical scavengers. Inhibition of the DCF fluorescence induced by PDGF (1 ng/mL) in situ is expressed as a function of time in the presence of catalase (1 mg/mL), N-acetylcysteine (NAC, 30 mM) or mannitol (Man, 100 μM). The data are expressed as the mean ± SD with n = 3. ♦, cells stimulated by 1 ng/mL PDGF alone with no preloading; ○, cells preloaded with N-acetylcysteine (NAC, 30 mM); ⟥, cells preloaded with catalase (Cat, 1 mg/mL); solid triangle ▲, cells preloaded with mannitol (Man,100 mM). Reprinted with permission from Chen et al., EER (2004); Copyright Elsevier 2004 [83].