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. 2022 Oct 21;11(10):2080. doi: 10.3390/antiox11102080

Figure 2.

Figure 2

Figure 2

Regulation of EMT- and MET-associated proteins by αBC-P in fetal hRPE cells. (a,b) Sub-confluent fetal RPE cells were treated with TGFβ2 (10 ng/mL) alone or/with Scr. (75 μg/mL) or/with αBC-P (50 and 75 μg/mL) or αBC-P (75 μg/mL) alone for 48 h. Protein expression was measured by Western blot analysis. EMT-associated proteins (αSMA, pSMAD2/3, SMAD2/3) and transcription proteins SNAIL and SLUG increased significantly with TGFβ2 stimulation, and αBC-P markedly inhibited their expression. Moreover, MET-associated protein (E-cadherin) decreased significantly with TGFβ2 stimulation, and αBC-P markedly increased their expression. (c) hRPE cells were cultured in 4 well chamber slides. The cells were treated with TGFβ2 (10 ng/mL) or/and αBC-P (75) for 24 h at 37 °C. After washing, cells were imaged with fluorescence microscope. Scale bar: 50 µm, (Left images) Red: αSMA, Green: E-cadherin, Blue: DAPI. (Right image) Red: αSMA, Green: E-cadherin, Blue: DAPI. Scr.: Scrambled peptide, αBC-P: αB crystallin-chaperone peptide. Values are means ± SEM. * p < 0.05, ** p < 0.01. n = 3.