Figure 5.

Increased respiration and ATP production in EMT and further activation by αBC-P. Mitochondrial bioenergetics were analyzed using Seahorse XFe96 (a). Sub-confluent RPE cells were treated with TGFβ2 (10 ng/mL) alone or/with αBC-P (25, 50, and 75 μg/mL) in DMEM containing 3% FBS for 48 h. TGFβ2-cotreated with αBC-P significantly increased mitochondrial bioenergetic parameters, such as basal respiration, Maximal Respiration, ATP production, and Spare Respiratory Capacity, compared with the TGFβ2-treated group in a dose-dependent manner (b). αBC-P: αB crystallin-chaperone peptide. Data normalized by μg/cellular protein. Values are means ± SEM. * p < 0.05, ** p < 0.01. n = 9–15.