Table 1.
Target Molecule | Accession Number | Forward Primer Sequence | Reverse Primer Sequence |
---|---|---|---|
αSMA | NM_001613 | 5′-TCTGTAAGGCCGGCTTTGC-3′ | 5′-TGTCCCATTCCCACCATCA-3′ |
Vimentin | NM_003380 | 5′-TGAGTACCGGAGACAGGTGCAG-3′ | 5′-TAGCAGCTTCAACGGCAAAGTTC-3′ |
E-cadherin | NM_004360 | 5′-ATTTTTCCCTCACACCCGAT-3′ | 5′-TCCCAGGCGTAGACCAAGA-3′ |
Sirt1 | NM_012238 | 5′-TCCTGGACAATTCCAGCCATCTCT-3′ | 5′-TTCCAGCGTTATGTTCTGGGT-3′ |
GAPDH | NM_002046 | 5′-ACAGTCGCCGCATCTTCTT-3′ | 5′-CTTGATTTTGGAGGGATCTCGC-3′ |
αSMA; α-smooth muscle actin, Sirt 1; sirtuin 1 GAPDH; glyceraldehyde-3-phosphate dehydrogenase.