Table 2.
List of Antibodies Used for Western Blot analysis.
| Target Molecule | Antibody Type | Source | Dilution |
|---|---|---|---|
| αSMA (ab7817) | Mouse monoclonal | Abcam | 1:1000 |
| E-cadherin (24E10) | Rabbit monoclonal | Cell Signaling | 1:1000 |
| pSMAD2/3 ((S465/467)/(S423/425) | Rabbit monoclonal | Cell Signaling | 1:1000 |
| SMAD2/3 (D7G7) | Rabbit monoclonal | Cell Signaling | 1:1000 |
| Fibronectin (EP5) | Mouse monoclonal | Santa Cruz Biotechnology | 1:1000 |
| Vimentin (3CB2) | Mouse monoclonal | Santa Cruz Biotechnology | 1:1000 |
| SNAIL (C15D3) | Rabbit monoclonal | Cell Signaling | 1:1000 |
| SLUG (G-18) | Goat polyclonal | Santa Cruz Biotechnology | 1:1000 |
| CTGF (ab6992) | Rabbit monoclonal | Abcam | 1:1000 |
| GAPDH (MAB374) | Mouse monoclonal | EMD Millipore | 1:1000 |
αSMA; α-smooth muscle actin, pSMAD2/3; phospho-smad2/smad3, SMAD2/3; smad2/smad3, CTGF; connective tissue growth factor, GAPDH; glyceraldehyde-3-phosphate dehydrogenase.