Table 4.
MS Concentration | Nitrogen Concentration | Number of Regenerating Explants (%) | Main Shoot Length (mm) | Mean for Medium | Main Shoot Weight (mg) | Mean for Medium |
---|---|---|---|---|---|---|
MS | 2N | 0 | - | - | - | |
1N | 0 | - | ||||
1/2N | 93 | 22.73 d * | 32.63 ab | |||
1/2 MS | 2N | 45 | 20.5 d | 23.56 C | 20.18 b | 31.86 A |
1N | 95 | 22.24 d | 29.39 b | |||
1/2N | 100 | 26.26 c | 39.66 a | |||
1/5 MS | 2N | 97.5 | 22.56 d | 27.96 B | 18.18 c | 26.15 BC |
1N | 60 | 31.77 ab | 31.84 ab | |||
1/2N | 100 | 29.50 bc | 28.35 b | |||
1/10 MS | 2N | 100 | 30.85 ac | 31.66 A | 29.12 b | 30.38 AB |
1N | 100 | 35.64 a | 32.27 ab | |||
1/2N | 100 | 28.49 bc | 29.75 b | |||
Mean for nitrogen concentration | 2N—22.91 B 1N—27.61 A 1/2N—26.75 A |
2N—20.64 C 1N—28.73 B 1/2N—32.62 A |
* Means followed by the same letter in columns do not differ significantly at α = 0.05. MS, 1/2MS, 1/5MS, 1/10MS represent the Murashige and Skoog medium concentration, 2N, 1N, and 1/2N represent the NH4NO3 + KNO3 concentration of the MS formulation.