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. 2022 Oct 8;11(10):1996. doi: 10.3390/antiox11101996

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Transcriptional regulation of stress-responsive genes by phlorizin. (A) GFP expression induced by either hsp-16.2 or sod-3 promoter was observed in a fluorescence microscope. (B) Quantification of fluorescence was determined using a fluorescence multi-reader. Relative percent expression is shown compared to 100% for untreated control. (C) Subcellular localization of DAF-16 fused to GFP was determined under a fluorescence microscope. (D) Percent of worms showing nuclear localization was compared between untreated control and phlorizin-treated group. Error bar indicates standard error. C, untreated control; PZ, phlorizin (10 μM); *, a significant difference (p < 0.05) compared to untreated control.