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. 2022 Oct 14;9(10):551. doi: 10.3390/bioengineering9100551

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Histological and immunohistological analysis of EHM-ring sections. HE staining of an EHM ring section showing even distribution of the cells throughout the EHM ring structure. EHM ring was formed using 1.5 mg/mL collagen concentration and 1000 CF cells/μL gel and was fixed and stained 24 h after casting. Darker dots implicate CF. Magnification 5× (A); or 20× (B). Bars represent 200 µm. Vimentin (green) and DAPI (blue) staining (C); and Z-stack image of CNA35 (red) and Vimentin (green) staining nuclei are blue (DAPI) (D) (bar represents 29.4 μm).