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The effect of 4 h (A); and 24 h (B) cyclic stretch (10%, 1 Hz) on gene expression in CF cultured on 2D monolayer Bioflex plates (Flex) (data from [32]) or 3D EHM fibers (EHM) conditions. Presented are the relative gene expression levels of Nppb, Tgfβ1, Ctgf and Acta2 in 2D (n = 5–12) and 3D (n = 6–9) in stretch (S) and non-stretch (NS) conditions. * p < 0.05; ** p < 0.01; *** p < 0.001. Bar indicates mean.
