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. 2022 Sep 20;11(10):1851. doi: 10.3390/antiox11101851

Figure 2.

Figure 2

QAE decreases inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated BV2 microglial cells. BV2 microglial cells were pretreated with QAE (25–100 μg/mL) for 3 h, and further stimulated with LPS (100 ng/mL) for (a) 12 h, (b) 8 h. (a) Protein expression of iNOS and COX-2. β-actin immunoblotting was used to verify equal protein loading (n = 3). (b) mRNA expression of iNOS (NOS2) and COX-2 (PTGS2). Relative gene expression was quantified by RT-qPCR, and normalized by mouse GAPDH expression of each sample (n = 4). Significant versus control, *** p < 0.001; Significant versus LPS alone-treated group, # p < 0.05, ## p < 0.01, ### p < 0.001.