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. 2022 Sep 20;11(10):1851. doi: 10.3390/antiox11101851

Figure 7.

Figure 7

Phytochemicals of QAE inhibited the expression of proinflammatory mediators. (a) Relative cell viability. BV2 microglial cells were treated with each phytochemical at indicated dose for 24 h. Cell viability was determined using CCK-8 (n = 4). (b) NO production. Cells were pretreated with each phytochemical (25–100 μM) for 3 h, and further exposed to LPS (100 ng/mL) for 24 h. Produced NO in media was determined using Griess reagent (n = 4). Relative mRNA expression of (c) iNOS (NOS2), COX-2 (PTGS2), and (d) TNF-α (TNF), IL-1β (IL1B), IL-6 (IL6), and MCP-1 (CCL2). Cells were pretreated with catechin (n = 4) or taxifolin (n = 3), and subsequently stimulated with LPS (100 ng/mL) for 8 h. Significant versus control, *** p < 0.001; Significant versus LPS alone-treated group, # p < 0.05, ## p < 0.01, ### p < 0.001.