Figure 6.

Hepatic mitochondrial function and efficiency, AMPK/ACC and CPT1 expression in control and BTBR mice. Hepatic mitochondrial respiration rates were evaluated with succinate + rotenone (A) or palmitoyl-carnitine + malate (B) as substrates. CPT activity was measured in isolated mitochondria (C). Mitochondrial oxygen consumption in the presence of oligomycin, uncoupled by carbonyl-cyanide-4(trifluoromethoxy)phenylhydrazone (FCCP) and the degree of coupling (D) were shown. Hepatic expression levels of phosphorylated adenosine-monophosphate-activated protein kinase-α (pAMPK), as ratio pAMPK/AMPK (E), phosphorylated acetyl-CoA-carboxylase (pACC) as ratio pACC/ACC, and carnitine-palmitoyl-transferase 1 (CPT-1) (F). n = 4. Data are presented as means ± SEM from n = 7 animals/group. * p < 0.05, ** p < 0.01, *** p < 0.001, BTBR vs. Control (CTR).