Table 2.
A comparison of CRP assays validated in veterinary medicine. Abbreviations: POCT—Point of Care Testing.
| Type of Test | Validation | Random-Access Availability | Duration | Prozone Effect | Lipemia and Hemolysis Interference | Inter-Assay Reliability (CV) |
|---|---|---|---|---|---|---|
| ELISA | [27,28] | − | Longer than acceptable for POCT | + | + [2] | 7.5–29% [27] |
| Immuno-turbidimetric | Canine-specific [29,30] POCT [27,28,31] |
+ | Short time | + | − [32] | <5% [32,33] <10% for all POCT, all 3 POCT correlated with the results from the ELISA reference method (0.97) but still did not achieve an acceptable coefficient [28,31] |
| Human-specific validated for use in canines [34,35] | + | Short time | − | Not stated | Every batch of reagents has to be calibrated for valid results; CV <10% [35] | |
| Colloidal gold immunochromatography | Species-specific rabbit anti-dog-CRP antibodies [36] | + | Short time | − | No interference in clinically relevant ranges of up to 800 mg/L bilirubin, 4 g/L hemoglobin and 8 g/L soybean oil (Intralipid) [36] |
moderate to high CRP concentrations, ≤11%; ≤28% at low concentrations [36] |
| Capillary reversed passive latex agglutination test | [37] | − | Longer than acceptable for POCT | − | Not stated | 10.28–12.40% [38] |
+ means present; − means absent; CV: coefficient of variation; Point-of-care tests: POCT.