Figure 1.

Genetic screening of a novel MAPKKK in SKN-1 transgenic C. elegans. (a) RNAi screening was performed in SKN-1 transgenic worms to identify MAPKKKs involved in the resistance to oxidative stress. Worms were fed with bacteria for each RNAi treatment 24 h before exposure to oxidative stress. Oxidative stress resistance assays were performed 16 h after RNAi, and the survival rate was calculated (n = 3). Data are shown as the mean ± SEM. * p < 0.05. All p values were calculated by ANOVA followed by Dunnett’s multiple comparison tests. (b) Oxidative stress resistance assay was performed on SKN-1 transgenic worms fed RNAi bacteria against mekk-3, nsy-1, or both mekk-3 and nsy-1 under treatment with 20 mM t-BOOH. Survival rate was calculated at the indicated time points (h). This representative experiment involved 30 worms in each group. Data are shown as the mean ± SEM.