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. 2022 Oct 18;11(10):1526. doi: 10.3390/biology11101526

Figure 2.

Figure 2

Expression of MEKK−3 in oxidative stress. (a) Localization of MEKK-3 labeled with GFP under normal conditions (left) and under treatment with 7.5 mM t-BOOH for 24 h (right). MEKK-3 transgenic worms were visualized by GFP expression using fluorescence microscopy. The white arrows indicate the MEKK-3::GFP protein in the intestine. (b) Quantification of MEKK-3::GFP was performed using the ImageJ software and summarized (n = 3). (c) Quantitative PCR analyses of mekk-3 (n = 3). Total RNA extraction from MEKK-3 transgenic worms was performed 24 h after the exposure to 7.5 mM t-BOOH. (d) Oxidative stress resistance assay for MEKK-3 transgenic worms. The survival rate was calculated at the indicated time points (h) after the exposure to 7.5 mM t-BOOH. This representative experiment involved 30 worms in each group. Data are shown as the mean ± SEM. * p < 0.05. ** p < 0.01. All p values were calculated by unpaired two-tailed Student’s t-tests.