Figure 6.

Effect of Dr-Nb on L-tyrosine nitrosylation induced from peroxynitrite, at pH 7.0 and 22.0 °C. (A) Dependence of the nitro-L-tyrosine yield on the Dr-Nb(III) (open squares) and apo-Dr-Nb (open circles) concentration in the absence of CO₂ (One-way ANOVA: open squares, **** p < 0.0001, 0 M versus 10 × 10⁻⁶ M, 20 × 10⁻⁶ M, and 30 × 10⁻⁶ M). (B) Dependence of the nitro-L-tyrosine yield on the Dr-Nb(III) (filled squares) and apo-Dr-Nb (filled circles) concentration in the presence of CO₂. The symbols on the y-axis (open and filled triangle) indicate the nitro-L-tyrosine yield in the absence and presence of CO₂, respectively. The L-tyrosine concentration was 1.0 × 10⁻⁴ M. The peroxynitrite concentration was 2.0 × 10⁻⁴ M. Where not shown, the standard deviation is smaller than the symbol.