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. 2022 Oct 20;12(10):1524. doi: 10.3390/biom12101524

Figure 1.

Figure 1

SMN has a granular appearance and progressively decreases during the second week of life in axons and motor nerve terminals of wild-type mice. (A). Maximum intensity projection in a representative neuromuscular preparation of the TVA muscle at P3. Immunofluorescence of endogenous SMN (Smn, white) is detected in motor axons and nerve terminals. Note the apposition of SMN with the postsynaptic marker BTX-rhodamine (red). Scale bar: 50 μm. (B). SMN (white) shows a granular pattern at the NMJ (left panels, scale bar: 10 μm), and axons (magenta, right panels, upper-scale bar: 5 µm, lower-scale bar: 3 µm). Note the single-file arrangement of the SMN granules in the thinner axons (inset) marked by anti-NF (green). (C). Frequency histogram of the spacing between axonal granules, as calculated by their intensity profile (inset). (D). The SMN signal (magenta) is intense at P3 and P6, weak at P8 and P10, and almost null at adulthood (P60) in the TVA muscle. Scale bars upper panels: 50 µm (P3–P10), 10 µm (P60); lower panels: 10 µm. (E). The graph displays the percent of the Smn area with respect to the postsynaptic area at different ages.