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. 2022 Oct 7;12(10):1437. doi: 10.3390/biom12101437

Figure 2.

Figure 2

Gentisic acid (GA) supplementation accelerates myelination in brain organoids: (A) Schematic representation of the timeline of brain organoid generation, including GA application at days 70–80. (B) Representative brightfield images document progression of organoid growth from iPSC to brain organoids. (C) RT-PCR analysis demonstrates significantly increased expression of oligodendrocyte (precursor) cell markers (NG2, OLIG2, and PDGFR) and genes related to myelin formation (MYRF and PLP) after GA supplementation when compared to conventionally generated brain organoids. * p < 0.05, ** p < 0.01, and *** p < 0.001 (D) Representative images display increased numbers of NG2+ and Olig2+ oligodendrocytes and increased expression of ABCA1 in GA supplemented brain organoids.