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. 2022 Sep 27;12(10):1385. doi: 10.3390/biom12101385

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A,B) Immunofluorescence staining of nasal polyps from patients with chronic rhinosinusitis with nasal polyps. Fluorescence images for MBP (red), galectin-10 (green), and DNA (blue) were obtained using a Carl Zeiss LSM780 confocal microscope, as previously described [36]. Intact eosinophils are massively infiltrated in polyps (Ai,Aii). The presence of ETotic eosinophils with extracellular traps (degenerated DNA), cell-free granules (punctate MBP), extracellular vesicles (punctate galectin-10), and CLCs (galectin-10-stained needle-like crystals; arrowheads) can be seen (Bi,Bii). Scale bars: 50 μm for (i) and 10 μm for (ii).