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. 2022 Oct 20;12(10):898. doi: 10.3390/bios12100898

Figure 1.

Figure 1

SARS-CoV-2 diagnosis by nucleic acid amplification method, qRT-PCR. (1,2). Nasopharyngeal swabs were taken from the patients. (3). RNA extracted/purified by RNA purification kit. (4). Extracted RNA is reverse transcribed to complementary DNA and amplification using the target-specific primers. (5). Quantitative real-time PCR (qRT-PCR) to identify the positive samples. Conducted to identify positive samples in real-time from the fluorescence of the dsDNA intercalating dyes such as SYBR green.