Skip to main content
. 2022 Oct 13;12(10):1471. doi: 10.3390/biom12101471

Figure 2.

Figure 2

The effect of genotype on the pain sensitivity determined by tail-flick test (A,B). The influence of genotype (A) and the influence of AAV-Cre injection (B) on thermal pain sensitivity. Data are the mean ± SEM. n = 5–14 (A), n = 8 (B). +/+, wild type; +/flox, floxed hetero; flox/flox, floxed homo. The effect of knockdown of RTP4 in PVN on the development of antinociceptive tolerance to morphine (C). The daily changes in antinociceptive effect of morphine during repeated administration. Data are the mean ± SEM. n = 7 (AAV-eGFP) (control), n = 10 (AAV-Cre), **** p < 0.0001 vs. Day1 (AAV-eGFP); # p < 0.05, #### p < 0.0001 vs. Day1 (AAV-Cre), Dunnett’s multiple comparison test; † p < 0.05, †† p < 0.01 vs. AAV-eGFP (control), repeated measures ANOVA and the post-hoc Tukey’s multiple comparisons test. The effect of AAV-Cre on RTP4 expression in PVN area (D and E). Representative images of the immunohistochemical signal for RTP4 and the AAV-eGFP signal of the virus infected in the PVN area. Arrows indicate the eGFP-positive cell. The mean intensity of the RTP4 signal in eGFP-positive cells were measured by Image J software, as described in the Methods section (E). The mean signal intensity of RTP4 in the RTP4/eGFP double-positive cells were measured from a single cryostat brain section, including the PVN region from 7 (AAV-eGFP) and 6 (AAV-Cre) mice. Ten to 40 eGFP-positive cells were measured from each section. Data are the mean ± SEM. n = 7 (AAV-eGFP) (control), n = 6 (AAV-Cre), * p < 0.05 unpaired t-test.