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. 2022 Oct 13;12(10):1471. doi: 10.3390/biom12101471

Figure 4.

Figure 4

The effect of inhibitors of signal transduction molecules on DAMGO-induced (24 h) increase in RTP4 mRNA levels (A–D). N2AMOPr cells (2 × 105 cells/well) were treated with DAMGO (10 µM) without or with pre-treatment, with inhibitors at the indicated concentrations for 24 h (PTX) (A) or 30 min (ActD (B), U0126 (C), Cal C (D), Pyridone 6 (E)) before addition of DAMGO. RT-qPCR was performed with specific primers against RTP4 and GAPDH. Data are the mean ± SEM. n = 5–31, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control (open bar); # p < 0.05, ## p < 0.01, ### p < 0.001 vs. DAMGO (closed bar), Tukey’s multiple comparisons test.