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Lyssavirus P3 proteins inhibit IFN-α-dependent nuclear accumulation of STAT1. (A and C) COS-7 cells expressing the indicated proteins were treated with (+ IFN-α) or without (no add.) IFN-α (1,000 U/mL, 0.5h) before fixation and immunostaining for STAT1 and analysis by CLSM. (B and D) Images such as those shown in A and C were analyzed to determine the Fn/c for STAT1 (mean ± SEM; n ≥ 88 cells from four independent assays). Scale bars = 5 μm.
