TABLE 2.

Cytokine/chemokine levels in BMDC transfected with different glycoprotein genes or infected with HSV-1^a

Cytokine or chemokine	Results after transfection with:									Results after infection with HSV-1
	gB	gC	gD	gE	gG	gH	gI	gK	gL	1 PFU	10 PFU
IL-1α	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑	↑↑↑↑
IL-1β	ns	ns	ns	ns	ns	ns	ns	ns	↑↑	↑↑↑↑	↑↑↑↑
IL-6	ns	ns	ns	↑	ns	ns	ns	ns	ns	↑↑↑↑	ns
IL-9	ns	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑
CXCL5 (LIX)	ns	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑↑
IL-17	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑↑	ns
CXCL10 (IP-10)	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑↑	↑↑↑
CXCL1 (KC)	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑	↑↑↑↑
CCL2 (MCP-1)	↑↑↑↑	ns	↑	↑↑	↑↑	↑↑	ns	ns	ns	↑↑↑	ns
CCL3 (MIP-1α)	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑↑	↑
CCL4 (MIP-1β)	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑↑	↑↑↑↑
M-CSF	ns	ns	ns	↑	ns	ns	ns	ns	ns	ns	ns
CXCL2 (MIP-2)	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑	↑↑↑↑
CXCL9 (MIG)	ns	ns	ns	↑↑	↑	ns	ns	ns	ns	↑↑	ns
CCL5 (RANTES)	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑	ns
VEGF	↑↑↑↑	ns	↑↑↑	↑↑↑↑	↑↑	↑↑↑↑	ns	ns	ns	ns	ns
TNF-α	ns	ns	ns	ns	ns	ns	ns	ns	ns	↑↑↑	↑↑↑

^aCytokine/chemokine levels in culture media were analyzed using mouse 32-plex panels and are shown as significant relative to vector-transfected cells. Experimental procedures are described in Materials and Methods. Briefly, BMDCs from naive mice were transfected with each glycoprotein plasmid or infected with 1 or 10 PFU/cell of HSV-1 strain McKrae. Transfected or infected cell supernatants were collected. Levels of G-CSF, eotaxin, GM-CSF, IFN-γ, IL-2, IL-4, IL-3, IL-5, IL-7, IL-10, IL-13, IL-12(p40), IL-12(p70), LIF, and IL-15 did not differ between control and transfected or infected cells and are not shown. ns, not significant relative to mock-transfected or mock-infected BMDCs. For all statistical tests, P values less than or equal to 0.05 were considered statistically significant and are marked by a single upward arrow (↑). Double upward arrows (↑↑) denote a P value of <0.01, three upward arrows (↑↑↑) equals a P value of <0.001, and four upward arrows (↑↑↑↑) a P value of <0.0001.