TABLE 3.

Three microbial interaction data types represented according to the proposed metadata guidelines in Table 2^a

Metadata	Example A	Example B	Example C
Microbial entities
participants	Azotobacter vinelandii DSM 85, Chlamydomonas reinhardtii strain 187, Alternaria sp. GYI-051221	Acidobacteria, Gammaproteobacteria	Bacteroides caccae ATCC 43185, Lactobacillus rhamnosus GG
	NCBI:txid354, NCBI:txid3055, NCBI:txid667197	NCBI:txid57723, NCBI:txid1236	NCBI:txid411901, NCBI:txid568703
sequence_id	N/A, N/A, FJ627005.1		NZ_CP022412.2, NZ_CP031290.1
env_origin		soil environment [ENVO:01001044], soil environment [ENVO:01001044]	digestive tract environment [ENVO:01001033], digestive tract environment [ENVO:01001033]
source_collection	commercial collection, commercial collection, isolation
Interaction inference methods
evidence_type	experimental evidence used in manual assertion [ECO:0000269]	high throughput evidence used in automatic assertion [ECO:0006057]	computational evidence [ECO:0007672]
method_type	cultivation-based, microscopy-based	sample-based	simulation-based
reference	https://doi.org/10.1007/s12223-010-0067-9	https://doi.org/10.1038/ismej.2011.119	https://doi.org/10.1038/nbt.3703
Interaction context
env_broad_scale		soil environment [ENVO:01001044]
compounds	chlorophyll [CHEBI:28966], cystathionine [CHEBI:17755]		alanine [CHEBI:16449], asparagine [CHEBI:22653], nicotinic acid [CHEBI:15940], lactate [CHEBI:24996]
medium_name	Azotobacter Medium [https://bacmedia.dsmz.de/medium/3]		DMEM 6429 (+ vitamin K, hemin, and arabinogalactan)
rel_to_oxygen	aerobic		anaerobic
temp	25		37
Interaction attributes
participants_outcome	1,1,1	N/Ab	1,1
ecological_outcome	cooperation	co-occurrence
strength		0.66
dependencies	contact

^aIncluded here are an interaction from an alga-bacteria- fungi cultivation experiment (A), a co-occurrence analysis of bacterial operational taxonomic units (B), and a genome-scale metabolic model (C).

^bNA, not applicable.