TABLE 1.

Primer sequences for qPCR analysis and amplicon sequencing^a

PCR target	Primer direction or probe	Sequence
qPCR
L. iners	Forward	AGTCTGCCTTGAAGATCGG
	Probe	FAM-CCAAGAGATCGGGATAACACCT
	Reverse	CTTTTAAACAGTTGATAGGCATCATC
L. crispatus	Forward	AACTAACAGATTTACTTCGGTAATGA
	Probe	ROX-CCCATAGTCTGGGATACCACTT
	Reverse	AGCTGATCATGCGATCTGC
Lactobacillaceae	Forward	ATGGAAGAACACCAGTGGCG
	Reverse	CAGCACTGAGAGGCGGAAAC
Amplicon sequencing (1 example; altered for other barcodes)
16S rRNA gene V4 region	Forward	CAAGCAGAAGACGGCATACGAGATAACTCTCGAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT
	Reverse	AATGATACGGCGACCACCGAGATCTACACATCGTACGTATGGTAATTGTGTGCCAGCMGCCGCGGTAA
ITS2	Forward	AATGATACGGCGACCACCGAGATCTACACATCGTACGGCCGGTCGAGTAGTGAATCATCGAATCTTTGAA
	Reverse	CAAGCAGAAGACGGCATACGAGATAACTCTCGAGTCAGTCAGGGTCCTCCGCTTATTGATATGC

^aPrimer sequences for qPCR analysis were obtained from previous studies by Kusters et al. (47) and Zariffard et al. (51), and amplicon sequencing was performed as described by Oerlemans et al. (24), adapted from methods described previously by Kozich et al. (52). FAM, 6-carboxyfluorescein; ROX, carboxyrhodamine.