TABLE 1.
Complex and defined media used in this study
| Media type | Component(s) | Product no. |
|---|---|---|
| NB | 8 g/L nutrient broth | RPI 50-488-846 |
| ISP1 | 5 g/L tryptone 3 g/L yeast extract |
BD 211705 Fisher BP1422 |
| ISP2 | 10 g/L malt extract 4 g/L yeast extract 4 g/L glucose |
BD 218630 Fisher BP1422 Fisher D16 |
| CAA | 2 g/L acid hydrolyzed casein | Fisher BP1424 |
| G-N-P-T | 55.5 mM glucose 10 mM (NH4)2SO4 25 mM potassium phosphate buffer (pH = 7.4) 1 mM MgSO4 100 nM CaCl2 100 nM NaCl 10 nM FeSO4 1 nM ZnSO4 1 nM MnSO4 1 nM CuSO4 |
Fisher D16 Fisher BP212R Fisher BP362 Fisher BP363 Fisher M65 Sigma C4901 Sigma S9888 Sigma 215422 Sigma 221376 Sigma M7634 Fisher BP346 |
| S-N-P-T | 5 g/L starch All compounds inG-N-P-T except glucose |
Sigma S9765 |
| AA-P | 5.6 g/L amino acid mix 25 mM potassium phosphate buffer (pH = 7.4) |
Teknova C0704 Fisher BP362 Fisher BP363 |