Skip to main content
. 2022 Sep 29;12(10):1388. doi: 10.3390/biom12101388

Figure 2.

Figure 2

The purification of PW-pGH28-3. Lane C, protein supernatant of E. coli BL21; Lane T, total protein mixture of E. coli BL21 expressed PW-pGH28-3; Lane S, supernatant protein mixture of E. coli BL21 expressed PW-pGH28-3; Lane P, precipitated protein mixture of E. coli BL21 expressed PW-pGH28-3; Lane 1, protein washed with 20 mM imidazole buffer; Lane 2, protein washed with 50 mM imidazole buffer; Lane 3, protein washed with 100 mM imidazole buffer; Lane 4, protein washed with 300 mM imidazole buffer for the first time; Lane 5, protein washed with 300 mM imidazole buffer for the second time; Lane M, molecular mass standards; Lane 6, protein washed with 500 mM imidazole buffer for the first time; Lane 7, protein washed with 500 mM imidazole buffer for the second time.