Table 1.
The 23 pectinolytic genes derived from the pulp and paper wastewater treatment microbiota and their closest genes in the GenBank database.
| Gene Name | Closest Genes | Accession Numbers |
Sequence Identity |
Endo- or Exo-Polygalacturonase Activity |
|---|---|---|---|---|
| PW-pGH28-1 | Hypothetical protein [Chloroflexi bacterium] | NLG99247.1 | 45.2% | |
| PW-pGH28-2 | Hypothetical protein [Dehalococcoidia bacterium] | NLE96346.1 | 69.9% | |
| PW-pGH28-3 | Exo- polygalacturonase [Aquipluma nitroreducens] | BBE19954.1 | 58.4% | ✓ |
| PW-pGH28-4 | Right-handed parallel beta-helix repeat-containing protein [Methanobacterium sp.] | MBI4813419.1 | 66.9% | |
| PW-pGH28-5 | Right-handed parallel beta-helix repeat-containing protein [Lentisphaerae bacterium] | NLC83012.1 | 94.8% | |
| PW-pGH28-6 | S-layer homology domain-containing protein [Armatimonadetes bacterium] | MBN1461142.1 | 49.5% | |
| PW-pGH28-7 | Glycoside hydrolase family 28 protein [Bacteroidales bacterium] | NLX28859.1 | 99.8% | |
| PW-pGH28-8 | Glycoside hydrolase family 28 protein [Bacteroidales bacterium] | MBN1107786.1 | 80.1% | ✓ |
| PW-pGH28-9 | Disaggregatase related [Methanomethylovorans sp. PtaU1.Bin073] | OPY19182.1 | 79.0% | |
| PW-pGH28-10 | Exo-poly-alpha-D-galacturonosidase [Bacteroidetes bacterium] | MBP1676534.1 | 76.4% | ✓ |
| PW-pGH28-11 | Right-handed parallel beta-helix repeat-containing protein [Bacteroidales bacterium] | MBG0861178.1 | 81.0% | ✓ |
| PW-pGH28-12 | Glycoside hydrolase family 28 protein [Paludibacter sp.] | MBP6662256.1 | 72.1% | |
| PW-pGH28-13 | TPA: hypothetical protein [Chloroflexi bacterium] | HFI27235.1 | 54.8% | |
| PW-pGH28-14 | Glycoside hydrolase family 28 protein [Bacteroidales bacterium] | MBK7713019.1 | 57.4% | |
| PW-pPL1-1 | T9SS type A sorting domain-containing protein [Catalinimonas alkaloidigena] | WP_089687757.1 | 52.1% | |
| PW-pPL1-2 | Pectate trisaccharide-lyase precursor [Bacteroidetes bacterium A Durb.BinA395] | OPZ03270.1 | 73.6% | ✓ |
| PW-pPL1-3 | Polysaccharide lyase [Bacteroides sp. 51] | WP_163173617.1 | 82.6% | |
| PW-pPL1-4 | Polysaccharide lyase [Paludibacter sp. SCN 50-10] | ODT57447.1 | 83.9% | |
| PW-pPL1-5 | Hypothetical protein BGP01_06875 [Paludibacter sp. 47-17] | OJX91998.1 | 57.5% | |
| PW-pPL1-6 | Pectate lyase [Phycisphaerae bacterium] | MBN2560505.1 | 80.7% | |
| PW-pPL1-7 | Hypothetical protein [Bacteroidetes bacterium] | MBP1676284.1 | 61.5% | |
| PW-pPL1-8 | Pectate lyase precursor [Deltaproteobacteria bacterium ] | MBM4340551.1 | 69.6% | |
| PW-pPL1-9 | Pectate lyase [Bacteroidales bacterium] | NLD63827.1 | 80.6% |