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. 2022 Sep 29;96(20):e01152-22. doi: 10.1128/jvi.01152-22

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Copyright © 2022 Hayward et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 11 — Expression of HA-tagged tetherin proteins. Human and Pteropus alecto tetherin isoforms A, B, and C in HEK293T cells. Tetherin localization in cells was detected using anti-HA-tag rabbit monoclonal Ig and anti-rabbit Alexa Fluor 488 secondary Ig. Nuclei are blue-stained with Hoechst. Fixed and permeabilized cells were imaged on a Nikon AR1 confocal microscope. (A) Human l-tetherin; (B) negative control, HEK293T cells treated without the inclusion of the primary antibody; (C) P. alecto tetherin isoform A; (D) P. alecto tetherin isoform B; (E) P. alecto tetherin isoform C. HA, hemagglutinin.