FIG 8.

Expression of alternative isoforms of tetherin in stimulated and unstimulated P. alecto spleen tissue. Spleen tissue from 15 individuals were unstimulated, or stimulated with lipopolysaccharide (LPS) or polyinosinic:poly(C) (PIC) (n = 5 for each treatment). Tetherin expression was determined from RNA-seq sequence reads mapped against the P. alecto genomic scaffold, KB030270, which harbors the tetherin gene. Tetherin expression levels are represented as normalized counts-per-million (CPM) reads. (A) Tetherin expression by isoform. P values were determined using a one-tailed Mann-Whitney U test. (B) Expression of tetherin isoform in each individual sample.