TABLE 4.
Predicted molecular mass for unglycosylated tetherin monomers and dimers
| Unglycosylated tetherin | Predicted molecular mass (kDa) |
|||||
|---|---|---|---|---|---|---|
| Human tetherin |
P. alecto
|
M. macropus
|
||||
| Tetherin iso A | Tetherin iso B | Tetherin iso Ca | Tetherin A | Tetherin B | ||
| Monomeric, HA-tagged, GSP-cleaved + GPI anchor | 20.8 | 20.1 | 19.4 | 22.1 | 21.3 | 14.5 |
| Dimeric, HA-tagged, GSP-cleaved + GPI anchor | 41.6 | 40.2 | 38.8 | 44.2 | 42.6 | 29 |
a
Molecular mass calculations for P. alecto tetherin isoform C do not involve GSP-cleavage or GPI-anchor addition. HA, hemagglutinin; GPI, glycophosphoinositol; GSP, GPI signal peptide.