TABLE 8.
qPCR primers and probes used to analyze tetherin expression in bat tissuesa
| Primer or probe | Target | Sequence (5′ > 3′)b | Direction |
|---|---|---|---|
| Primers | |||
| Teth_F | Tetherin | TGACTGTGGCCGTGATCGT | Forward |
| Teth_R | CCATTTTTGCAGGCCTCACT | Reverse | |
| 18S_rRNA_F | 18S rRNA | CGGCTACCACATCCAAGGAA | Forward |
| 18S_rRNA_R | GCTGGAATTACCGCGGCT | Reverse | |
| Probes | |||
| Teth_Probe | Tetherin | FAM_TCGCCGTCGAGAACA_MGB | NA |
| 18S_Probe | 18S rRNA | VIC_TGCTGGCACCAGACTTGCCCTC_TAMRA | NA |
a
qPCR, quantitative PCR; NA, not applicable.
b
Probe 5′ reporters and 3′ quenchers are indicated with italics.