Figure 1.

Relationship between TRAF3 status and inactive GSK3β in B cell lymphoma cell lines. (A) Cells from BCL cell lines were lysed and GSK3β or TRAF3 was immunoprecipitated. IP samples and input/whole cell lysate (WCL) were analyzed by Western blotting. (B) Representative blot of TRAF3, GSK3β and pGSK3β^S9 in a panel of BCL cell lines. (C) Graph of amount of TRAF3 vs. ratio of inactive (pGSK3β):total GSK3β from data in (B). (D) Western blot of human B cells from the peripheral blood of healthy donors. (E) Graph of amount of TRAF3 vs. ratio of inactive (pGSK3β):total GSK3β from data in (F). Representative Western blot of pGSK3β^S9 and total GSK3β in primary mouse B cells from mice with or without a B cell-specific TRAF3 deficiency. (G) Graph of amount of TRAF3 vs. ratio of inactive (pGSK3β):total GSK3β from data in (F). (H) Quantification of blots in (D). Blots are representative of at least 3 independent experiments (A,B). In (C), each lane represents an individual human donor. In (D), each lane represents an individual mouse. n.s., not significant by student’s t-test. Uncropped blots and densitometry shown in Figures S3 and S4.