Figure 2.

TRAF3 status of BCL cell lines affects susceptibility to GSK3 inhibition. (A) Survival of BCL cell lines after 48 h incubation with indicated concentrations of 9-ING-41 (left) and IC₅₀ calculated from survival data (right). (B) Survival of WT or Traf3^−/− primary mouse B cells after 24 or 48 h incubation with indicated concentrations of 9-ING-41. In right two graphs, cells were activated with LPS 2 h prior to initiation of 9-ING-41 treatment. (C) Survival of BCL cell line BJAB and two clones of TRAF3-deficient BJAB after 48 h incubation with indicated concentrations of 9-ING-41. Graphed are mean ± SEM for at least three independent replicates. **** p < 0.0001 by repeated measures ANOVA with Sidak’s multiple comparisons test.