Figure 1.

Sequences of wild-type YSK₂ and the three constructs. (A) Multiple sequence alignment of YSK₂, YSK₂K→R, YSK₂-SpaceK, and YSK₂-Neutral. The alignment was performed using ClustalW and the image was created using ESPript. Conserved regions are shown bounded by a blue box. White letters on a red background show residues conserved among all sequences, while red letters on a white background show residues that are similar. (B) Distribution of lysine and negatively charged residues in the YSK₂-SpaceK and YSK₂-Neutral constructs. All negatively charged residues are shown in red and all lysine residues are shown in blue.