Figure 6.

The Relationship between Yfh1 helicity and construct concentration. The CD spectrum of 10 μM Yfh1 was determined at 1 °C in 10 mM Tris, pH 7.4, in the presence and absence of the various dehydrin constructs. The equation described in Section 2 was used to find the percent α-helicity of Yfh1. Three replicates were used to create this image and the standard deviation is represented by the error bars. The dashed line indicates the helicity of Yfh1 alone at 25 °C, while the dotted line indicates the helicity of Yfh1 alone at 1 °C. YSK₂, light blue line; YSK₂-SpaceK, red line; YSK₂-Neutral, green line; YSK₂-K→R, yellow line.