FIG 3.
AltL is responsible for C28 transport. (A) Induced expression of gfp gene in strains ΔalmA/alkMa::gfp-pKRG and ΔaltL/almA/alkMa::gfp-pKRG in the presence of C28. The expression levels of gfp in ΔalmA/alkMa::gfp-pKRG was normalized to 1. (B) GFP fluorescence in strains ΔalmA/alkMa::gfp-pKRG and ΔaltL/almA/alkMa::gfp-pKRG in the presence of C28. GFP fluorescence was calculated as relative fluorescence units (F/OD), which represent fluorescence values per OD600. Values are shown as means ± SD (n = 3) and were analyzed by independent-sample t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant). (C) Growth curves of strains WT, ΔaltL, and ΔaltL/PaltL cultured in SA medium supplemented with C28 at 30°C. The inset shows the growth of strains in the initial 3 h of culture. (D) Changes in δ13CV-PDB value for strains WT, ΔaltL, and △altL/PaltL cultured in SA medium supplemented with C28-1,2-13C2 at 30°C measured at different times. The inset shows the changes of δ13CV-PDB value in the initial 8 h of culture. SA represents BSM medium supplemented with 0.5% sodium acetate, and SA+C28 represents BSM medium supplemented with 0.5% sodium acetate and 0.1% C28. Values are from one independent experiment.